Neurotransmitter Receptors, Transporters, and Ion Channels
Calcineurin A/PPP3CA
Product Name
Calcineurin A/PPP3CA Protein, Human, Recombinant (His Tag)
Synonyms
CALN Protein, Human; CALNA Protein, Human; CALNA1 Protein, Human; CCN1 Protein, Human; CNA1 Protein, Human; PPP2B Protein, Human
Purity
> 94 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Using the Octet RED System, the affinity constant (Kd) of human PPPP3CA-His (Cat:13670-H07B) bound to Human PPIA-His (Cat:10436-H08E) was 0.9 nM.
Protein Construction
A DNA sequence encoding the human PPP3CA (Q08209-1) (Ser2-Gln521) was fused with a polyhistide tag at the N-terminus.
Expressed Host
Baculovirus-Insect Cells
Molecule Mass
The recombinant human PPP3CA consists of 538 amino acids and has a calculated molecular mass of 60.8 kDa. The recombinant protein migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerolPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
