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CaMKII beta/CAMK2B Protein, Human, Recombinant (His & GST Tag)

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Specification

Cat. No.

NRT009

Neurotransmitter Receptors, Transporters, and Ion Channels

CaMKII beta/CAMK2B

Product Type

Proteins

Product Name

CaMKII beta/CAMK2B Protein, Human, Recombinant (His & GST Tag)

Size

50 µg

Price

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Synonyms

CAM2 Protein, Human; CAMK2 Protein, Human; CAMKB Protein, Human

Purity

> 60 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

The specific activity was determined to be 300 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate.

Protein Construction

A DNA sequence encoding the human CAMK2B isoform 2 (Q13554-3) (Met 1-Gln 503) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Accession#

Q13554-3

Expressed Host

Baculovirus-Insect Cells

Species

Human

Predicted N Terminal

Met

Molecule Mass

The recombinant human CAMK2B/GST chimera consists of 740 amino acids and has a calculated molecular mass of 84.2 kDa. It migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.

Formulation

Supplied as sterile 20mM Tris, 500mM NaCl, 2mM GSH, 0.5mM PMSF, 10% gly, pH 8.0Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

Shipping

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.