Neurotransmitter Receptors, Transporters, and Ion Channels
DCLK1
Product Name
DCLK1 Protein, Human, Recombinant (aa 1-705, His & GST Tag)
Synonyms
CL1 Protein, Human; CLICK1 Protein, Human; DCAMKL1 Protein, Human; DCDC3A Protein, Human; DCLK Protein, Human
Purity
> 84 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
The specific activity was determined to be >2.0 nmol/min/mg using synthetic Autocamtide-2 peptide (KKALRRQETVDAL-amide) as substrate.
Protein Construction
A DNA sequence encoding the human DCLK1 (O15075-1) (Met 1-Val 705) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host
Baculovirus-Insect Cells
Molecule Mass
The recombinant human DCLK1/GST chimera consists of 942 amino acids and has a calculated molecular mass of 106 KDa. It migrates as an approximately 105 KDa band as predicted in SDS-PAGE under reducing conditions.
Formulation
Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 0.5mM PMSFPlease contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
