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- BBB DamageCategories
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- Ionotropic Receptor AntibodiesMetabotropic Receptor AntibodiesNeurotransmitter AntibodiesTransporter AntibodiesSignal Channel Antibodies
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- Glial Cell Marker AntibodiesNeural Stem Cell Marker AntibodiesNeuron Marker AntibodiesSynaptic Marker AntibodiesCell Death and ApoptosisNeuroinflammationNeuronal Damage and RepairOxidative StressVascular Mechanisms
- 3D BBB Models
- Organ-on-a-Chip BBB ModelsTranswell Models3D BBB Models Culture Substrate
- 3D HydrogelsCollagensAnmial BBB ModelsCell Lines for BBB Modeling
- Immortalized CellsPrimary CellsStem Cells
Cell Lines for BBB Modeling
As the most basic unit in biological research, cells have been widely used as the vital study tool of basic research, drug screening, disease models and tissue engineering, thereby providing an important evidence for drug development and treatment. Although traditional animal models can more comprehensively simulate the complexity and specificity of human diseases, they are not easy to conduct a large number of repeated experiments due to the 3R principle and animal ethics issues. Compared with animal models, cell models, as a simple, fast and economical research tool, provide researchers with more opportunities to initially explore the mechanisms of disease and conduct large-scale drug screening.
BBB Models
The blood-brain barrier (BBB) refers to an extremely important histological barrier located in the intima of cerebral blood vessels, which composed of special cells including astrocytes, vascular endothelial cells and pericytes to generate neurovascular units and thereby involved in the regulation of neuronal activity. With the advancement of science and technology, more and more cell lines are used to construct in vitro BBB models. There are several cell lines that are commonly used for modeling the BBB.
- Brain capillary endothelial cell line (BCEC): The BCEC cell line is derived from brain capillary endothelial cells, which can form tight junctions and express various transporters and enzymes in the blood-brain barrier.
- Brain microvascular endothelial cell line (BMEC): The BMEC cell line is derived from brain microvascular endothelial cells and has been shown to exhibit multiple characteristics of the blood-brain barrier and can be used alone to create BBB models, such as human brain microvascular endothelial cell line (HBMEC).
- Astrocyte-derived cell lines: Astrocytes play a critical role in maintaining the integrity and function of the blood-brain barrier and are often used in combination with BCEC or BMEC cell lines to model the BBB.
- Pericyte-derived cell lines: Pericytes are another important cell type in the blood-brain barrier and are also commonly used in combination with BCEC or BMEC cell lines to model the BBB.
Ace Therapeutics provides hundreds of species, different types of cell lines that can be used alone or in combination to construct the models of BBB to study the behavior, structure and function of the blood-brain barrier, as well as for the development of drug delivery and treatment strategies for neurological diseases.
In addition to single cell lines, we provide multiple 3D coculture BBB models to more accurately represent the BBB, which typically involve a combination of endothelial cells (BCECs or BMECs) with other supporting cells such as astrocytes or pericytes.
Click on the Links to Access Our Cell Lines for BBB Modeling:
Cat.# Name Description Price BBM-C-122 Human Brain Microvascular Endothelial Cells Human Brain Microvascular Endothelial Cells (HBMECs), derived from the human brain, are a specific type of brain endothelial cells that play a crucial role in forming the blood-brain barrier (BBB). They prevent the entry of soluble and cellular substances from the blood into the brain and protect it against toxins and immune cells. These brain microvascular endothelial cells have the ability to display cell adhesion molecules on their surface, which control the migration of leukocytes into the brain. Nevertheless, when there are host-pathogen interactions outside the cells, it can activate calcium signaling within the endothelial cells, leading to the penetration of pathogens through the BBB. Human Brain Microvascular Endothelial Cells from Ace Therapeutics are isolated from normal human brain cortical tissue. Each vial contains at least 5×10^5 cells per ml and is delivered frozen.inquiry BBM-C-123 Human Brain Microvascular Pericytes Human brain microvascular pericytes (HBMVPCs) perform numerous tasks which include playing a vital part in constructing and maintaining the blood-brain barrier, controlling angiogenesis, ensuring endothelial cell survival, and engulfing cellular waste. Human Brain Microvascular Pericytes from Ace Therapeutics are isolated from normal human brain cortical tissue. Each vial contains at least 5×10^5 cells per ml and is delivered frozen.inquiry BBM-C-124 Human Astrocytes Human Astrocytes from Ace Therapeutics are isolated from the human cerebral cortex, cryopreserved at passage one. Each vial contains at least 5×10^5 cells per ml and is delivered frozen.inquiry BBM-C-125 CD1 Mouse Brain Microvascular Endothelial Cells CD1 Mouse Brain Microvascular Endothelial Cells from Ace Therapeutics are isolated from brain tissue of pathogen-free laboratory mice. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-126 C57BL/6 Mouse Embryonic Brain Endothelial Cells C57BL/6 Mouse Embryonic Brain Endothelial Cells from Ace Therapeutics are isolated from brain tissue of pathogen-free laboratory mice at embryonic day 14.5-19. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-127 C57BL/6 Mouse Brain Microvascular Endothelial Cells C57BL/6 mouse brain endothelial cells (MBREC) from Ace Therapeutics are isolated from the brain tissue of pathogen-free laboratory mice. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-128 BALB/c Mouse Brain Microvascular Endothelial Cells BALB/c Mouse Brain Microvascular Endothelial Cells from Ace Therapeutics are isolated from the brain tissue of pathogen-free laboratory mice. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-129 Rat Brain Microvascular Endothelial Cells Rat Primary Brain Microvascular Endothelial Cells (RPBECs) from Ace Therapeutics are isolated from the brain tissue of 1-day-old neonatal Sprague-Dawley rats. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-130 Bovine Brain Microvascular Endothelial Cells Bovine Brain Microvascular Endothelial Cells (BBMVEC) from Ace Therapeutics are isolated from microvessels of the bovine brain. The cells are cryopreserved at the second passage.inquiry BBM-C-131 Cynomolgus Monkey Brain Microvascular Endothelial Cells Cynomolgus Monkey Brain Microvascular Endothelial Cells from Ace Therapeutics are isolated from Cynomolgus monkey brain tissue. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-132 Porcine Brain Microvascular Endothelial Cells Porcine Brain Microvascular Endothelial Cells from Ace Therapeutics are isolated from healthy porcine brain tissue. Porcine Brain Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with a gelatin-based solution for 0.5 hours and incubated in Ace Therapeutics’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-133 Canine Brain Microvascular Endothelial Cells Canine Brain Microvascular Endothelial Cells from Ace Therapeutics are isolated from the brain of a pathogen-free laboratory beagle dog. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.inquiry BBM-C-134 Rabbit Brain Microvascular Endothelial Cells Rabbit Brain Microvascular Endothelial Cells from Ace Therapeutics are isolated from the brain tissue of a pathogen-free New Zealand White Rabbit. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-135 Mouse Brain Vascular Pericytes (MBVP) Mouse Brain Vascular Pericytes (MBVP) from Ace Therapeutics are isolated from adult mouse brains and cryopreserved at P2. MBVP express Neural/glial antigen 2 (NG2) and α-smooth muscle actin. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-136 Rat Brain Vascular Pericytes (RBVP) Rat Brain Vascular Pericytes (RBVP) from Ace Therapeutics are isolated from adult rat brains and cryopreserved at P2. MBVP expresses Neural/glial antigen 2 (NG2) and α-smooth muscle actin. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry All of our products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.0Inquiry Basket