Custom Collagenase Model of Intracerebral Hemorrhage in Animals

Intracerebral hemorrhage (ICH), which accounts for 10-20% of all strokes, has been modeled in the laboratory by intracerebral injection of autologous blood, injection of embolus, or proteolysis of the basal lamina surrounding brain capillaries by bacterial collagenase. The choice of model depends on the pathophysiological concept underlying the hemorrhage. Collagenase is a metalloprotease that degrades type IV collagen in the basal lamina of the blood-brain barrier, ultimately leading to microvascular rupture and leakage around the needle site. The collagenase injection model requires the injection of various amounts (0.01-0.1 units in 0.5-2 mL) of bacterial collagenase into the rat striatum.

Fig. 1. Collagenase-induced intracerebral hemorrhage in a murine model. (Lauer et al., 2013)

Our Custom Collagenase Model of Intracerebral Hemorrhage in Animals

Ace Therapeutics offers collagenase injection-induced ICH models to support your preclinical research on hemorrhagic stroke.

Animal Modeling Method

The basic step in the construction of our collagenase injection model is the stereotaxic injection of 0.075U bacterial (Clostridia) collagenase dissolved in 0.5 μL saline into brain regions of rats or mice, causing specific brain parenchymal or intraventricular hemorrhage. We can also design a custom package with the amount of collagenase injected based on the severity of the hematoma. Our expert team is well-versed in stereotactic-assisted surgery, ensuring precise and reproducible injections of blood or collagenase into targeted brain areas.

Animal Model Characterization and Validation

Our laboratory can evaluate morphological and behavioral changes in animals with collagenase-induced ICH to ensure adequate performance of the procedure, or to investigate the effects of potential treatments.

• Histological appearance of hematoma: Hematoxylin and eosin (H&E) staining
• Behavioral assessment: Corner turning and forelimb placement test
• Measurement of cerebral edema: Quantifying the extent of brain damage after experimental intracerebral hemorrhage

Advantages of Our Custom Collagenase Injection Stroke Model

• Our collagenase injection hemorrhagic stroke model is highly reproducible.
• We can control the size of the hematoma by adjusting the amount of collagenase.

Applications

• Study treatments after ICH
• Study the effects of hematoma and cerebral edema

At Ace Therapeutics, our collagenase injection-induced ICH model provides a more comprehensive approach to support your hemorrhagic stroke preclinical research. Need to know more? Contact us and let one of our experts provide you with all the answers you need.

1. Lauer, A., et al. (2013). Intracerebral hemorrhage associated with antithrombotic treatment: translational insights from experimental studies. The Lancet Neurology, 12(4), 394-405.