Exploration of the Role of Blood Brain Barrier in Stroke

The blood brain barrier (BBB) is a characteristic structure of microvessels in the brain that prevents harmful substances from entering the brain parenchyma in the central nervous system. At the same time, it will interact with the disease pathological process. Stroke affects the BBB until it breaks down through inflammation, oxidative stress, immunity, and other mechanisms. However, the deeper mechanism of action is still unclear. Therefore, Ace Therapeutics provides a series of services to explore the impact of BBB on the pathological process of stroke.

Exploration of the Role of Different Proteins in Blood Brain Barrier in Stroke

Many proteins have been found to influence the BBB during stroke pathologies, such as cytokines, chemokines, adhesion molecules, immunoglobulin superfamily, integrins, and other proteins. They affect the pathological process of stroke by regulating a variety of physiological and pathological pathways.

• We have established in vitro and in vivo models of BBB disruption to explore the changes of different proteins in the BBB.
• We use gene microarray and protein microarray technology to find the differential genes and proteins in the damaged BBB.
• To explore the effect of the different gene or protein expression abnormalities on the pathological process of stroke, we have established stroke models with different gene expression abnormalities by drug administration or gene knockout technology.
• We quantify and localize differential genes and proteins by qPCR, western blotting, ELISA, and immunohistochemistry to explore their specific roles in the BBB.

Exploration of the Role of Different Cell Types in Blood Brain Barrier in Stroke

Studies have shown that endothelial cells, astrocytes, pericytes, microglia, leukocytes, lymphocytes, and a variety of other cells are involved in the regulation of BBB. They regulate the pathological changes of BBB and the pathological process of stroke by affecting immunity, inflammation, BBB structure, and others.

• We perform the pathological diagnosis of the blood brain barrier in stroke and analyze the specific cell types and damage.
• We screen differential genes and proteins by gene chip and protein chip technology.
• To explore and validate the effect of abnormal gene expression in different cells on stroke models, we have established in vitro and in vivo stroke models with abnormal gene expression by drug administration or gene knockout technology.
• We detect the specific mechanisms of the differential genes and proteins with qPCR, ELISA, western blotting, immunohistochemistry, and other assays.

You are free to customize the testing service you need or let our professional scientists optimize and implement the complete experimental method for you. If you would like to learn more about our services, please feel free to contact us.

1. Yang, C., et al. Neuroinflammatory mechanisms of blood-brain barrier damage in ischemic stroke. Am J Physiol Cell Physiol. 2019, 316(2): p.C135-c153.