Exploration of the Role of Cell Apoptosis in Stroke

Many causes contribute to the development of cell apoptosis during stroke pathologies, such as ATP depletion and free radicals leading to cell apoptosis of neurons and glial cells, calcium influx leading to cell apoptosis of neurons and oligodendrocytes, and inflammatory response and α-syn aggregation inducing cell apoptosis of neurons. Although the end result is the occurrence of cell apoptosis, the pathological processes that occur in different conditions are quite different. That can also reflect, to some extent, the pathological processes experienced in stroke. Therefore, Ace Therapeutics offers comprehensive services to explore the role of cell apoptosis in the pathological process of stroke.

Cell Apoptosis Identification Services

Ace Therapeutics provides high - quality services to identify cell apoptosis during stroke pathology.

• We perform the morphological examination of cells by fluorescence confocal laser scanning microscopy and transmission electron microscopy.
• We detect early cell apoptosis by Annexin V / PI double staining.
• We offer mitochondrial membrane potential assay, Tunel assay, DNA fragmentation assay, and apoptosis factor assay services.

Exploration of the Role of Mitochondria - Mediated Cell Apoptosis in Stroke

During the stroke, calcium overload leads to mitochondrial dysfunction, which in turn leads to the development of cell apoptosis. In view of this, Ace Therapeutics provides comprehensive services to explore mitochondrial - mediated cell apoptosis.

• We detect changes in mitochondrial transmembrane potential after cell apoptosis by mitochondrial membrane potential.
• We perform qualitative, quantitative, and localization assays for genes and proteins of the B - cell lymphoma - 2 (Bcl - 2) family by PCR, western blotting, ELISA, immunohistochemistry, and other assays.
• We screen for differential proteins in related processes by gene microarray and protein microarray techniques to identify their role in mitochondria-mediated cell apoptosis during stroke pathology.

Exploration of the Role of Endoplasmic Reticulum - Mediated Cell Apoptosis in Stroke

Dysregulation of calcium homeostasis leads to the accumulation and aggregation of unfolded proteins, resulting in a severe endoplasmic reticulum stress response and the appearance of endoplasmic reticulum-mediated cell apoptosis. However, this apoptotic pathway has been less studied in stroke pathology. Therefore, Ace Therapeutics provides comprehensive biological services to explore its role in the pathology of stroke.

• We explore the role of the IRE1 signaling pathway, PERK signaling pathway, and caspase7 signaling pathway in the apoptotic process during stroke pathology by PCR, western blotting, ELISA, and immunofluorescence assays.
• We explore the differential targets in endoplasmic reticulum-induced cell apoptosis by gene microarray and protein microarray techniques.

Exploration of the Role of Death Receptor Pathway - Mediated Cell Apoptosis in Stroke

The death receptor pathway is initiated by external factors and induces cell apoptosis. Ace Therapeutics provides comprehensive services to explore the specific role of death receptor pathway-mediated cell apoptosis in stroke pathology.

• We offer a comprehensive range of assay services targeting proteins of various death receptor pathways.
• We use gene microarrays and protein microarrays to screen for downstream genes and proteins of the death receptor pathway to obtain differential targets.

Ace Therapeutics offers a one-stop scientific service plat to explore the role of cell apoptosis in stroke in a scientific and accurate manner. If you would like to learn more about our services, please feel free to contact us.

1. Sekerdag, E., et al., Cell death mechanisms in stroke and novel molecular and cellular treatment options. Curr Neuropharmacol, 2018. 16(9): p. 1396-1415.