Exploration of the Role of Exosomes in Stroke

Exosomes are involved in many pathological processes in strokes, such as angiogenesis, neurogenesis, anti-apoptotic effects, autophagy, and regulation of the blood brain barrier. Studies have shown that exosomes can effectively promote brain repair after stroke. Therefore, Exploration of the Role of exosomes in stroke is helpful to accelerate people's understanding of stroke and promote the development of exosomes in stroke therapy. Ace Therapeutics provides comprehensive services to explore the role of exosomes in stroke.

Exploration of The Neurogenesis Effect of Exosomes in Stroke

Exosomes are transmitted through biological fluids and enable communication between adjacent and remote cells. Therefore, exosomes are involved in regulating many physiological and pathological processes, including nerve and vascular regeneration. Exosomes derived from mesenchymal stem cell-Schwann cells and macrophages can accelerate peripheral nerve regeneration. In addition, exosomes promote neural cell proliferation and migration, alleviate neuroinflammation, and other activities through different pathways. Ace Therapeutics provides comprehensive services to explore the effects of exosomes on neurogenesis in stroke.

• We provide different in vitro and in vivo stroke models to explore the role of exosomes in post-stroke neurogenesis.
• We assess neurogenesis by measuring neurons and their proliferation and migration.
• We test the effect of exosomes on inflammation in the pathological process of stroke by detecting proinflammatory factors and inflammasomes.

Exploration of The Angiogenesis Effect of Exosomes in Stroke

Exosomes act as intercellular signals to neuroregulate cerebrovascular integrity, which has been proved to be a new way of neurovascular communication. Studies have shown that exosomes induced by MSCs can activate and promote endogenous angiogenesis. Therefore, Ace Therapeutics provides comprehensive services to explore the effect of exosomes on angiogenesis during stroke pathology.

• The degree of angiogenesis is identified by measuring the migration and proliferation of endothelial cells and vascular morphogenesis.
• Specific proteins in angiogenesis, such as VEGF-C, are detected by PCR, western blotting, and ELISA.
• The metabolism of endothelial cells is measured by tracer metabolomics, radiotracer-based metabolic assays, extracellular acidification rate, and mitochondrial respiration measured by hippocampal XF analyzer.

Exploration of The Antiapoptotic Effect of Exosomes in Stroke

Previous studies have shown that exosomes can regulate the apoptosis process in the pathological process of stroke by regulating apoptotic factors. Meanwhile, exosomes can regulate the caspase-8-dependent apoptosis pathway. Given the important role of exosomes in regulating apoptosis in stroke, Ace Therapeutics provides comprehensive services to explore the effect of exosomes on apoptosis by regulating the pathological process of stroke.

• We have established in vitro and in vivo stroke models to evaluate the role of exosomes in the apoptotic process of stroke.
• We evaluate the effect of exosomes on neuronal apoptosis by detecting cell activity.
• The specific characteristics of apoptosis are detected by optical microscopy, transmission electron microscopy, and laser confocal microscopy.
• We detect apoptotic body, Bcl-2 family proteins, and caspase family by PCR and western blotting assays.

If you would like to learn more about our services, please feel free to contact us.

1. Wang, M. M., et al., The role of exosomes in stroke. Mol Biol Rep, 2020. 47(8): p. 6217-6228.