Exploration of the Role of Heat Shock Protein 70 in Stroke

After the onset of stroke, HSP70 is rapidly upregulated in response to various pathological processes. It regulates the pathological process of stroke mainly by mediating cell death and inflammatory responses. However, more mechanisms of action are not yet clear. Even the results of some studies show that HSP70 has no protective effect in transgenic stroke mouse models. Given the ambiguity and inconsistency of the current results on HSP70, Ace Therapeutics provides a comprehensive and rigorous service to explore the role of HSP70 in the stroke process.

Exploration of the Role of HSP70 in The Cell Death Signaling Pathway in Stroke

HSP70 can regulate a variety of cell death-related signaling pathways, including the intrinsic apoptotic pathway mediated by Bcl-2 family proteins and the extrinsic apoptotic pathway mediated by cell surface receptors. To address the role of HSP70 in cell death signaling pathways, we provide different stroke models and a well-established assay service.

• We evaluate the role of HSP70 in cell death signaling through in vitro and in vivo stroke models by HSP70 overexpression or deficiency, such as manipulation of HSP70 expression by viral vector-mediated overexpression, siRNA-mediated downregulation, or by induction of sublethal heat stress.
• We investigate Bcl-2 family proteins by western blotting, ELISA, immunohistochemistry, and immunofluorescence assays.
• We observe cell morphology by fluorescence confocal laser scanning microscopy and transmission electron microscopy, Annexin V /PI double staining method, mitochondrial membrane potential assay, activated caspase-3 assay, DNA fragmentation assay, and other methods to detect the status of apoptosis.

Exploration of the Role of HSP70 in The Inflammation in Stroke

Neuroinflammation is a key feature of acute nerve injury and an important pathological activity in stroke. Studies have proved that in the pathological process of stroke, HSP70 can enhance the immune response outside the cell and regulate the inflammatory response inside the cell, but how to regulate the inflammatory response is still unclear. Ace Therapeutics provides a series of services to explore the role of HSP70 in the regulation of inflammatory response in the pathological process of stroke.

• In view of the regulatory effect of HSP70 on NF-κB, MAPK, and other signaling pathways, we provide western blotting and ELISA to quantitatively and localize NF-κB and MAPK signaling pathways and their downstream pathways.
• In view of the regulatory effect of HSP70 on oxidative stress process, we detected the expression of ROS, NO, iNOS and other active molecules in oxidative stress process by ELISA and biochemical reagent detection.

Ace Therapeutics provides a one-stop scientific service to explore the role of HSP70 in stroke in a scientific and accurate manner. If you would like to learn more about our services, please feel free to contact us.

1. Kim, J.Y., et al. The 70-kda heat shock protein (hsp70) as a therapeutic target for stroke. Expert Opin Ther Targets. 2018, 22(3): p.191-199.