Exploration of the Role of Leukocytes in Stroke

Leukocytes are one of the immunocompetent cells involved in stroke, and their activation and recruitment are key stages in the initiation and maintenance of neuroinflammation. Although many studies have been performed to explore the specific role of leukocytes in stroke, the understanding of the mechanisms of action of leukocyte activation and function after stroke is still very limited. In turn, a clear understanding of the mechanisms of action behind leukocyte function is essential for the development of new strategies for the treatment of stroke. Therefore, Ace Therapeutics offers comprehensive services to explore the specific role played by leukocytes in the pathological process of stroke.

Exploration of the Role of Neutrophils in Stroke

Neutrophils are the first blood-derived immune cells to reach ischemic brain tissue. It reacts rapidly within 15 min after the onset of ischemic stroke by binding various adhesion molecules, peaking 2-4 days after stroke and decreasing thereafter. Neutrophils play an important role throughout the stroke onset. However, the exact mechanism of action is not well understood. Ace Therapeutics provides comprehensive services to explore the mechanisms of neutrophil action during the stroke.

• To establish in vitro and in vivo models of stroke.
• To establish stroke models with gene deletion or overexpression, such as different adhesion factors, to investigate the effect of abnormal expression of target genes on stroke.
• To test the immune response of the body after stroke and to detect the expression levels of adhesion factors such as ICAM-1, MAC-1, and selectins.
• To detect the integrity of the blood brain barrier, detect the size of the infarct, and monitor the development of the pathological process after stroke.

Exploration of the Role of Monocytes in Strok

Monocytes are incompletely differentiated cells, highly phagocytic, whose response depends on the nature of the stimulus in their microenvironment. By expressing specific surface markers, the phenotype of monocytes can be divided into pro-inflammatory and anti-inflammatory subpopulations. It has been found that monocytes are able to participate in the inflammatory response, angiogenesis, and other processes in the damaged brain through different pathways. Ace Therapeutics provides comprehensive services to explore the specific mechanisms of the role of monocytes in stroke pathology.

• We offer microfluidic cell culture systems to refine studies related to angiogenesis.
• We detect the inflammatory response of the stroke process involving monocytes by PCR, ELISA, and western blotting.
• The effect of monocytes on angiogenesis is evaluated by measuring the migration and proliferation of endothelial cells.
• The effect of monocytes on angiogenesis is evaluated by detecting specific markers such as vascular endothelial growth factor c and vascular endothelial growth factor 3.

Exploration of the Role of Lymphocytes in Stroke

Studies suggest that lymphocytes may negatively contribute to the pathogenesis of post-stroke brain injury through the release of pro-inflammatory cytokines and cytotoxic substances. In contrast, blocking the entry of lymphocytes into infarcted brain tissue may reduce the extent of the injury. In addition, T lymphocytes are a key factor in the expansion of inflammation after stroke. Given the potentially deleterious role of lymphocytes in the stroke process, Ace Therapeutics provides comprehensive services to explore the mechanisms of lymphocyte action in the stroke process.

• To construct in vivo stroke models with different lymphocyte deficiencies, study the effect of lymphocyte deficiency on stroke, and identify the effect of different types of lymphocyte deficiency in stroke.
• To analyze and identify key pro-inflammatory factors and cytotoxic substances by PCR, ELISA, western blotting, and other assays to detect the secretion of pro-inflammatory factors and other cytotoxic substances.

If you would like to learn more about our services, please feel free to contact us.

1. Jian, Z., et al., The involvement and therapy target of immune cells after ischemic stroke. Front Immunol, 2019. 10: p. 2167.