Exploration of the Role of Microglia in Stroke

Activation of microglia induces the production of many mediators that mediate the different pathological processes of stroke. Due to the diversity of mediators, they also exert different effects on different pathological processes in the stroke process. A clinical study on stroke found that, on the one hand, activated microglia in the infarct zone can exacerbate delayed neuronal death by producing toxic substances. On the other hand, activated microglia promote neuronal regeneration by producing growth factors. However, it is not clear about the specific role of microglia in stroke. Therefore, Ace Therapeutics provides comprehensive services to explore the role of microglia in stroke pathology from different perspectives by combining current studies.

Exploration of the Role of Activated Microglia in Stroke

Many factors have the ability to activate microglia, such as Toll-like receptors, histamine and substance P, and programmed cell death protein 1. In contrast, activated microglia disrupt blood-brain barrier permeability and enhance apoptosis of astrocytes. Ace Therapeutics provides comprehensive services to detect the effects of activated microglia on the stroke process.

• We establish microglia activation models by different activation modalities in order to investigate how different microglia activation can have different effects on the pathological process of stroke.
• We evaluate the permeability of the BBB in vitro model by cell permeability assay and transendothelial resistance assay.
• We evaluate the permeability of the BBB in vitro model by detecting the expression of biomarkers, such as tight junction proteins, SLCs transporters, and ABC transporters.
• We evaluate the role of activated microglia on oxidative stress during the stroke by detecting the level of ROS.

Exploration of the Role of Different Phenotypes of Microglia in Stroke

In response to stimulation by anti-inflammatory and pro-inflammatory factors, microglia can generally polarize into inflammatory phenotype (M1 phenotype) and anti-inflammatory phenotype (M2 phenotype). M1 phenotype produces a variety of pro-inflammatory factors such as IL-1β, IL-6, TNF-α, CCL2, CXCL10, and others. It is generally believed that the M1 phenotype promotes inflammatory responses while the M2 phenotype has protective effects. Ace Therapeutics provides a comprehensive service to explore the role of different phenotypes of microglia in the stroke process.

• We determine the effect of different phenotypes of microglia on the stroke process and the related signaling pathways by protein microarrays or PCR microarrays.
• For the different signaling pathways involved in the regulation of microglia polarization in the stroke process, we clarify the changes of key molecules in the signaling pathway by qPCR, western blotting, ELISA, and other assays.
• Further studies of key molecules, including molecular co-expression, molecular interactions, and molecular localization, are performed to clarify the specific mechanisms of molecular actions.

Ace Therapeutics provides a one-stop scientific service to explore the role of microglia in stroke in a scientific and accurate manner. If you would like to learn more about our services, please feel free to contact us.

1. Zhao, S. C., et al., Regulation of microglial activation in stroke. Acta Pharmacol Sin, 2017. 38(4): p. 445-458.
2. Yenari, M. A., et al., Microglial activation in stroke: Therapeutic targets. Neurotherapeutics, 2010. 7(4): p. 378-391.