Exploration of the Role of Noncoding RNAs in Stroke

Little is still known about the specific mechanism of action of non-coding RNAs (ncRNAs) in stroke, but studies have confirmed that ncRNAs can regulate the pathological process of stroke by controlling processes such as transcription and translation. The overall research on drugs targeting ncRNAs has progressed rapidly, but related research in the field of stroke has been slow, in large part because the role of ncRNAs in stroke is not yet known. To address this issue, Ace Therapeutics offers professional services to accelerate your research exploring the role of ncRNAs in stroke.

Exploration of the Role of miRNAs in Stroke

It was found that miRNAs can be used as a biomarker of stroke and can be used to identify ischemic stroke and hemorrhagic stroke. However, its specific role in stroke is not yet known. Therefore, Ace Therapeutics offers a series of services to explore the role of miRNAs in stroke.

• By using microarray technology and next-generation sequencing technology, we can initially screen the differentially expressed miRNAs in stroke models.
• The initial screening results are validated by the qRT-PCR technique, northern blotting, and other assays.
• Based on the stroke model with altered miRNAs expression levels, we analyze the role of miRNAs in stroke by techniques such as miRNAs target gene prediction, Gene Ontology functional significance enrichment analysis, and pathway significance enrichment analysis.

Exploration of the Role of lncRNAs in Stroke

lncRNAs affect the pathological process of stroke through various pathways such as apoptosis and inflammation. However, the related mechanisms of action are not in-depth, so Ace Therapeutics provides a comprehensive service to explore the role of lncRNAs in stroke.

• We provide QD-FISH in situ hybridization technique and qRT-PCR technique to detect the distribution of lncRNAs. Meanwhile, we provide the full-length sequence of lncRNAs detection service.
• By performing function gain or deletion of lncRNAs and then detecting its effect on stroke models, we have successfully established methods for the construction of lncRNAs overexpression plasmids or lentiviral and adenoviral packaging vectors and various methods for silencing lncRNAs to verify the effect of lncRNAs on stroke by qRT-PCR or FISH.
• We provide in vitro transcription, RNA pull-down, RNA-RIP (RNA binding protein immunoprecipitation), CHIRP-seq (Chromatin isolation by RNA purification), MS, and other assays to explore the relationship between lncRNAs and protein, DNA and RNA interactions.

Exploration of the Role of circRNAs in Stroke

circRNAs are associated with multiple pathological pathways in the brain during strokes, such as neuronal apoptosis, excitotoxicity, neuroinflammation, and autophagy. However, the specific roles of circRNAs in these processes are not known. Therefore, Ace Therapeutics provides a comprehensive service to explore the role of circRNAs in the pathological processes of stroke.

• We have established a high-throughput circRNAs detection platform that screens for key circRNAs associated with stroke.
• We explore the role of circRNAs in the stroke process by disrupting or overexpressing them.
• We validate and quantify circRNAs by RT-PCR, RT-qPCR, and northern blotting.
• We validate the roles and connections of circRNAs-related signaling pathways based on RNA pull-down, RIP, FISH co-localization, EMSA experimental expression profiling, and GSEA analysis.

If you would like to learn more about our services, please feel free to contact us.

1. Chen, M., et al., Long non-coding rnas and circular rnas: Insights into microglia and astrocyte mediated neurological diseases. Front Mol Neurosci, 2021. 14: p. 745066.
2. Zhang, L., et al., Circular rna in acute central nervous system injuries: A new target for therapeutic intervention. Front Mol Neurosci, 2022. 15: p. 816182.
3. Ouyang, Y. B., et al., Micrornas: Innovative targets for cerebral ischemia and stroke. Curr Drug Targets, 2013. 14(1): p. 90-101.