Exploration of the Role of Notch in Stroke

Notch is a membrane receptor that regulates cell proliferation, differentiation and developmental fate switching in a range of tissues, and participates in physiological and pathological processes. Notch protein is activated by brain ischemia and hypoxia in stroke. Four prominent pathways have been identified, namely NF-κB, p53, HIF-1α, and Pin1. In addition, Notch has many other roles in stroke, such as neurogenesis, nerve cell regulation, and inflammatory processes. Ace Therapeutics provides a series of services to explore the role of Notch in stroke.

Exploration of the Role of Notch in Cell Death in Stroke

The notch signaling pathway is involved in the regulation of cell survival and death. In the pathological process of stroke, Notch mediates mastermind-like (MAML), HATp300, Hairy-Enhancer of Split (HES), HES-related protein (HERP), and other proteins to regulate the process of cell death. Given the important role of Notch, Ace Therapeutics provides comprehensive services to explore the Notch-mediated cell death process in the pathological process of stroke.

• We provide different cell death models of stroke in vitro and in vivo, such as abnormal Notch expression, to explore the mechanism of Notch action in the pathological process of stroke.
• Based on the experimental model, we screen the differential genes and proteins in Notch-mediated stroke by gene chip and protein chip technology.
• We examine the role of differential genes and proteins in cell death during Notch-mediated stroke by qPCR, ELISA, western blotting, co-immunoprecipitation, pull-down, and other assays.
• Different assays are developed to identify different cell death types and evaluate cell viability.

Exploration of the Role of Notch / NF-κB / p53 / HIF-1α Axis in Stroke

Studies have shown that the Notch / NF-κB / p53 / HIF-1α axis is involved in the regulation of stroke pathological processes, such as cell stress response, neuronal adaptive response to bioenergetic challenges, and the promotion of neuronal survival during recovery and remodeling phases after ischemic stroke. Ace Therapeutics provides comprehensive services to explore the role of the Notch / NF-κB / p53 / HIF-1α axis in the pathological process of stroke.

• In order to investigate the role of different proteins in the Notch / NF-κB / p53 / HIF-1α axis in stroke, we have established various experimental models of abnormal expression of different proteins by drug administration and gene manipulation.
• We screen the key pathways of the Notch / NF-κB / p53 / HIF-1α axis in the stroke process by gene chip and protein chip technology.
• We detect the specific mechanisms of the differential genes and proteins with qPCR, ELISA, western blotting, immunohistochemistry, and other assays.

You are free to customize the testing services you need or let our professional scientists optimize and implement the complete experimental methods for you. If you would like to learn more about our services, please feel free to contact us.

1. Arumugam, T.V., et al. Notch signaling and neuronal death in stroke. Prog Neurobiol. 2018, 165-167(p.103-116.12827-12843.