Exploration of the Role of Nrf2 in Stroke

Studies have shown that Nrf2 has antioxidant, anti-inflammatory, and regulatory activities in heme and iron metabolism, and that it is an important cofactor for many drug-metabolizing enzymes and antioxidants that mediate a variety of physiological and pathological processes in vivo. The current study provides a preliminary understanding of the antioxidant and anti-inflammatory activities of Nrf2 in stroke pathology, but the exact mechanism of action is not yet clear. Therefore, Ace Therapeutics provides comprehensive services to investigate the role of Nrf2 in stroke pathology.

Exploration of The Neuroprotective Role of Nrf2 in Stroke

Given the role of Nrf2 in oxidative stress and inflammatory responses, Ace Therapeutics provides quality services to explore the role played by Nrf2 in stroke pathology.

• We have established in vitro and in vivo different stroke models to explore the role of Nrf2 in the pathological process of stroke.
• We have established in vitro and in vivo models of abnormal Nrf2 expression through drug administration and genetic manipulation.
• For animal models, we provide pathology services to detect brain damage in animals after a stroke.
• For animal models, we provide comprehensive behavioral evaluation services to measure behavioral, cognitive, and other aspects of the animals' abilities after a stroke.

Exploration of the Role of Nrf2 in The Oxidative Stress Response in Stroke

Given the ability of Nrf2 to precisely counteract oxidative stress at multiple levels, as well as the multiple pathways through which Nrf2 counteracts oxidative stress to exert neuroprotective effects during ischemic stroke pathology, Ace Therapeutics provides comprehensive services to explore the role played by the antioxidant activity of Nrf2 during the stroke pathology.

• Given that Nrf2 can regulate oxidative stress processes related to glutathione, thioredoxin, G6PDH, and 6PGD, we perform gene microarray and protein microarray screening for these four oxidative stress-related pathways to identify differential targets.
• For the differential targets, we provide qualitative, quantitative, and localization assays to initially understand the action of the differential targets, and then investigate their specific mechanisms of action by means of protein interactions.
• For other oxidative stress pathways, we also provide a comprehensive service to explore them. We can screen for different oxidative stress responses and investigate the specific role of the antioxidant stress activity of Nrf2 in the pathology of stroke.

Exploration of the Role of Nrf2 in The Inflammatory Response in Stroke

To address the anti-inflammatory activity of Nrf2, we have comprehensively explored its role in the stroke process from different perspectives.

• We explore the differential targets of Nrf2-associated inflammatory pathways during stroke pathology by protein microarray.
• We perform in-depth studies of the differential targets by ELISA, PCR, western blotting, immunohistochemistry, and other modalities.
• We quantify and localize different inflammatory factors in stroke models with aberrant Nrf2 expression.

For other roles of Nrf2 in stroke pathology, we likewise offer comprehensive exploration services, such as clarifying the brain sites where Nrf2 acts, the brain cells associated with Nrf2 activity, and other aspects. If you would like to learn more about our services, please feel free to contact us.

1. Farina, M., et al., The nrf2 pathway in ischemic stroke: A review. Molecules, 2021. 26(16).