Exploration of the Role of Pyroptosis in Stroke

Pyroptosis is closely related to inflammation. During the pathological process of stroke, pyroptosis triggers an inflammatory response and exacerbates brain tissue damage through an increase in NLRP1 or NLRP3 inflammatory vesicles or caspase-1. Pyroptosis releases large amounts of cellular contents and pro-inflammatory factors that induce a cascade amplification of the inflammatory response during the stroke. Therefore, it is important to explore the role of pyroptosis in stroke, especially the role of inflammation regulation, in order to unravel the mystery of stroke at an early stage. With this in mind, Ace Therapeutics offers a range of services to explore the role of pyroptosis in stroke in all aspects.

Exploration of the Role of Inflammasome in Pyroptosis in Stroke

Inflammasome assembly is a key part of pyroptosis and inflammation during cerebral ischemia. Inflammasome plays an important role in pyroptosis, inflammation, and immune response. Therefore, it plays an important role in the pathology of stroke. Ace Therapeutics provides a series of services to explore the role of the inflammasome in pyroptosis during a stroke.

• We offer different pathways to detect the activation of the inflammasome. We detect the expression level of the inflammasome by rt-PCR, monitor ASC spot formation in cells by fluorescence microscopy or flow cytometry, detect caspase-1 cleavage by western blotting, and measure the release of IL-1β, IL-18, or HMGB1 by ELISA.
• We quantify and localize NLRP3 in microglia and endothelial cells by immunofluorescence, immunoblotting, and immunocolloidal gold labeling techniques.
• We detect the release of pro-inflammatory cytokines by ELISA, western blotting, immunospotting, and Meso Scale Discovery and cytometric bead array assay platforms.

Exploration of the Role of Caspases in Pyroptosis in Stroke

Pyroptosis is an inflammatory caspase-induced lytic cell death, and caspase -1, -4, -5, and -11 are all effective in driving pyroptosis. Tight regulation of inflammatory caspase function and pyroptosis is necessary to escape the dysregulated neuroinflammation and stroke pathophysiological consequences. Therefore, Ace Therapeutics offers a comprehensive service to explore the role of caspases in the pathology of stroke.

• We provide protein microarray and PCR microarray technologies to screen key molecules in the caspase-1-dependent classical pathway and caspase-4, 5, 11-dependent non-classical pathway during stroke.
• We identify the changes of key molecules during the stroke by western blotting, ELISA, and qPCR assays.
• We further clarify the specific mode of action of key molecules in stroke by examining their molecular interactions, molecular localization, and molecular conformation.

Exploration of the Role of GSDMD in Pyroptosis in Stroke

As the executor of thermal cleavage, the cleavage of GSDMD has an important impact on the occurrence of pyroptosis and the resulting inflammatory response. It is crucial to explore the role of GSDMD in regulating the pathological process of stroke by regulating pyroptosis. Therefore, Ace Therapeutics offers a range of services to explore the role of GSDMD in pyroptosis in stroke.

• We have successfully established in vitro and in vivo experimental models of aberrant GSDMD expression through drug administration and gene manipulation.
• We detect and analyze the changes and effects of downstream signaling pathways of GSDMD by western blotting, ELISA, rt-PCR, and other assays.

Ace Therapeutics offers a one-stop scientific service to explore the role of pyroptosis in stroke in a scientific and accurate manner. If you would like to learn more about our services, please feel free to contact us.

1. Kesavardhana, S., et al., Caspases in cell death, inflammation, and pyroptosis. Annu Rev Immunol, 2020. 38: p. 567-595.
2. Gou, X., et al., Pyroptosis in stroke-new insights into disease mechanisms and therapeutic strategies. J Physiol Biochem, 2021. 77(4): p. 511-529.