Exploration of the Role of UCHL1 in Stroke

It was found that the expression level of ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) has significant changes during ischemic stroke. Further studies suggest that UCHL1 is a potential biomarker of ischemic stroke. It is involved in regulating and maintaining brain function through multiple pathways. On the one hand, it is an important component of the ubiquitin proteasome pathway (UPP), which removes abnormal proteins from neurons while preventing the accumulation of toxic proteins. On the other hand, it participates in regulating oxidative damage, neuroinflammation, and motor pathways. However, its specific mechanism of action in stroke pathology is unclear. Therefore, in order to explore the specific role of UCHL1 in stroke pathology, Ace Therapeutics provides comprehensive services to promote the understanding of stroke and also to facilitate the development of stroke therapies targeting UCHL1.

Exploration of the Role of UCHL1 in UPP in Stroke

Given that ischemic stroke can impair UPP function through the production of reactive oxygen species such as nitric oxide, superoxide, and reactive lipid species such as isoprostanes, levogranidin, and cyclopentenone prostaglandins, and other pathways. However, the role played by UCHL1, a major component of the UPP, is not well understood. Therefore, Ace Therapeutics provides comprehensive services to explore the role played by UCHL1 as a major component of UPP in ischemic stroke.

• We provide different models of ischemic stroke to explore the specific mechanism of action of UCHL1 in different ischemic stroke conditions.
• We screen for differential biomarkers of UCHL1 during UPP by means of overexpression or gene silencing of UCHL1 and combining gene microarrays and protein microarrays.
• We provide further insight into the role of differential biomarkers in UPP with aberrant UCHL1 expression by qualitative, quantitative, and localization assays.
• We explore the interaction of differential biomarkers with UCHL1 by protein interaction techniques.

Exploration of Other Roles of UCHL1 in Stroke

In view of the complex function of UCHL1 in stroke, Ace Therapeutics also provides comprehensive services to explore in depth its other functions in stroke.

• To address the regulation of stroke response by reactive lipids through modification of UCHL1, we provide comprehensive assays for post-translational modification of UCHL1 to explore the UCHL1 modification process.
• For UCHL1-mediated oxidative stress in the stroke process, we provide comprehensive oxidative stress assays to comprehensively cover the needs of different studies.

You are free to customize the testing services you need or let our professional scientists optimize and implement the complete experimental methods for you. If you would like to learn more about our services, please feel free to contact us.

1. Graham, S. H.Liu, H., Life and death in the trash heap: The ubiquitin proteasome pathway and uchl1 in brain aging, neurodegenerative disease and cerebral ischemia. Ageing Res Rev, 2017. 34: p. 30-38.