Stroke Drug Development Targeting Notch

Notch is related to the regulation of many cell life activities, such as cell death, cell proliferation and differentiation, and developmental fate switching. In stroke, Notch mediates neurogenesis, cell survival and death, inflammatory response, and oxidative stress. In view of the important role of Notch in the pathological process of stroke, targeting Notch to develop stroke drugs is an important way to treat stroke. Therefore, Ace Therapeutics provides comprehensive services to study the development of stroke drugs targeting Notch.

Target Identification & Characterization

Notch regulates cell survival, death, proliferation, differentiation, and other processes through different signaling pathways. Therefore, it is an effective direction for stroke drug development to screen targets of related pathways by regulating Notch and then develop modulators with potential therapeutic activity against these targets. Ace Therapeutics provides comprehensive services to screen specific Notch-related targets in stroke pathology.

• We use genomics, proteomics, and metabolomics platforms to perform high-throughput screening of differential genes and proteins in Notch-related pathways.
• In vitro and in vivo stroke models with abnormal Notch expression are established by drug administration and genetic manipulation, which are used as vectors to screen candidate targets.

Screening of Notch Modulators

In view of the important regulatory role of Notch in various cellular activities, effective screening of appropriate modulators to regulate Notch activity is an important way to develop stroke drugs against candidate Notch pathway targets. Therefore, Ace Therapeutics has established different screening platforms for potential stroke drug candidates based on candidate Notch pathway targets.

• We have established in vitro and in vivo experimental models of abnormal-expression Notch by drug administration and genetic manipulation, which can be used as carriers for high-throughput screening and validation targeting Notch.
• Based on our small molecule library, including ultra-large compound library, natural product library, and fragment library, we have established different high throughput screening platforms targeting Notch.

Preclinical Evaluation of Drug Candidates for the Treatment of Stroke

The preclinical evaluation of drug candidates is an important step in drug development. For identified Notch modulators, Ace Therapeutics provides a well-developed service to examine their effects on stroke.

• We provide a variety of in vitro and in vivo stroke models to evaluate the therapeutic effects of candidate modulators on stroke.
• We perform gene chip and protein chip detection in stroke models with abnormal Notch expression to screen the related signaling pathways.
• We quantify and localize differential genes and proteins by qPCR, ELISA, western blotting, immunohistochemistry, co-immunoprecipitation, and other assays to determine the mechanism of action of drug candidates in regulating and treating the stroke process.

If you would like to learn more about our services, please feel free to contact us.

1. Arumugam, T.V., et al. Notch signaling and neuronal death in stroke. Prog Neurobiol. 2018, 165-167(p.103-116.12827-12843.