Category
Neural Stem Cell Growth Factors
Product Name
EGFL7 Protein, Human, Recombinant (His Tag)
Synonyms
NEU1 Protein, Human; VE-STATIN Protein, Human; ZNEU1 Protein, Human
Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
1. Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 × 10E4 cells/well areadded to EGFL7-His coated plates (1.25μg/mL and 100μL/well),approximately >45% will adhere specifically after 30 minutes at 37℃.
Protein Construction
A DNA sequence encoding the mature form of human EGFL7 (NP_057299.1) (Tyr 24-Ser 273) was expressed, with a polyhistidine tag at the N-terminus.
Expressed Host
Baculovirus-Insect Cells
Molecule Mass
The recombinant human EGFL7 consists of 268 amino acids and predicts a molecular mass of 29.4 kDa. It migrates as an approximately 31 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 100mM Glycine, 10mM NaCl, 10% glycerol, pH 7.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
