Exploration of the Role of AMPK in Stroke

AMPK plays an important role as a cellular energy sensor in the function of energy metabolism. It has been found that AMPK can influence multiple aspects of stroke, especially ischemic stroke pathology, including oxidative stress, autophagy, cell apoptosis, mitochondrial dysfunction, glutamate excitotoxicity, neuroinflammation, endothelial cells, and angiogenesis. It is now generally accepted that AMPK acts as a protective molecule in the stroke process, but remains controversial. Ace Neuroscience provides comprehensive services to elucidate the specific mechanisms of AMPK action in stroke pathology.

Exploration of The Beneficial Effects of AMPK on Stroke

For the different beneficial roles of AMPK during stroke pathology, Ace Neuroscience offers targeted exploration services.

• For the role of AMPK in protecting against ischemic stroke by preventing brain damage during oxidative stress, we provide different assays for malondialdehyde, ketone bodies, and other biomarkers.
• For the role of AMPK pre-activation in promoting neurological autophagy and ameliorating ischemic injury, we provide comprehensive assays for cellular autophagy, such as observation of morphological changes in different stages of autophagy by transmission electron microscopy, and detection of LC3, p62 and other biomarkers by western blotting, fluorescence indicator system, and other assays.
• For the regulatory role of AMPK on cell apoptosis in stroke pathology, we provide brain cell activity assay services and apoptosis-related biomarker testing services.
• For the role of AMPK in the regulation of mitochondrial function during stroke pathology, we offer brain cell activity assays, apoptosis-related biomarkers assays, such as mitochondrial fission factor, type 2 cannabinoid receptor, proliferation-activated receptor gamma coactivator-1α, NRF-1, and TFAM.

Exploration of The Deleterious Effects of AMPK on Stroke

Although AMPK has shown a protective effect against ischemic stroke in most studies, there remain many studies demonstrating that inhibition of AMPK activity is a potential pathway for stroke treatment in some cases. However, there are no definitive studies on the deleterious effects of AMPK on stroke. In view of this, Ace Neuroscience offers comprehensive services to explore the deleterious role of AMPK in the pathological process of stroke.

• We offer different stroke models with abnormal AMPK expression for studying the impact of AMPK in stroke pathological processes.
• We offer gene microarray and protein microarray technologies to screen for differential targets of AMPK-associated stroke pathological processes.
• We screen the pathways through which AMPK contributes to the pathological process of stroke by different techniques.
• For the pathways to be tested, we examine the specific mechanisms of action of the pathways to be tested by western blotting, ELISA, immunofluorescence, and protein interactions techniques.

In addition, Ace Neuroscience provides a one-stop scientific service to explore the role of AMPK in stroke in a scientific and accurate manner. If you would like to learn more about our services, please feel free to contact us.

1. Jiang, S., et al., Ampk: Potential therapeutic target for ischemic stroke. Theranostics, 2018. 8(16): p. 4535-4551.