Exploration of the Role of Epigenetic Mechanisms in Stroke

Epigenetics is an important branch of molecular biology, including DNA methylation, histone modification, and non-coding RNAs. Studies have found that epigenetics is involved in the pathological processes of many diseases, such as diabetes, obesity, renal pathology, and several types of cancer. The mechanism of action of ncRNAs has been explored separately, so the role of DNA methylation and histone modifications in stroke pathology is highlighted here. Ace Neuroscience provides comprehensive services to explore the role of epigenetic mechanisms in stroke.

Exploration of the Role of Histone Modifications in Stroke

The status of histone modification will affect the pathological process of various diseases, especially tumor, and immune diseases. Of these, acetylation and methylation are the most common types of histone modifications, and acetylation has also been shown to play an important role in stroke. Therefore, Ace Neuroscience provides perfect services to explore the specific mechanisms of different histone modification types during the stroke.

• The samples are processed by bottom-up, middle-down, and top-down assays for mass spectrometry analysis.
• We use protein microarray technology to detect histone modifications with high throughput.
• The status of histone modifications is determined by chromatin immunoprecipitation, western blotting, and detection of histone acetylase and deacetylase activities.

Exploration of the Role of DNA Methylation in Stroke

With the development of research, the role of DNA methylation in stroke has been paid more and more attention. Despite several studies related to the role of DNA methylation in stroke, it remains unclear. Ace Neuroscience provides comprehensive services to explore the role of DNA methylation in stroke.

• We combine DNA methylation with next-generation sequencing to build different DNA methylation sequencing platforms to meet your different sample sequencing needs.
• As an important enzyme of DNA methylation, DNA methyltransferase (DNMT) plays an important role in the pathological process of stroke. We have established in vitro and in vivo models of DNA methyltransferases with different gene function loss by gene knockout, gene mutation, and other technologies, in order to study the effects of DNA methyltransferases with different gene function loss on DNA methylation process in the pathological process of stroke.
• We combine methylomics and transcriptomic association analysis of DNA methylation to screen for key differential genes in the stroke process. In addition, we use qPCR, western blotting, ELISA, and other assays to explore the specific mechanism of key genes in stroke.

Click Exploration of the Role of Noncoding RNAs in Stroke to learn about the services of Exploration of the Role of noncoding RNAs in stroke. If you would like to learn more about our services, please feel free to contact us.

1. Stanzione, R., et al. Pathogenesis of ischemic stroke: Role of epigenetic mechanisms. Genes (Basel). 2020, 11(1): 12827-12843.